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KMID : 0043319920150030229
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Archives of Pharmacal Research
1992 Volume.15 No. 3 p.229 ~ p.233
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Determination of the pKa for Histidine-51 Residue in the Ternary Complex of Horse Liver Alcohol Dehydrogenase
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Lee KM
Son SY
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Abstract
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The pKa value of histidine-51 residue was determined by the pH dependency of contents of NADH bound to the active site in the horse liver alcohol dehydrogenase and % inactivation with diethyl pyrocarbonate treatment of the enzyme. The pKa for His-51 was ~7.15 in the ternary complex and ~6.7 in the enzyme itself.
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